pGusXD C386
Ligation of GusD-Hind-Sal* into pOCS-BM*
- Promoter 2
- MCS
- Gene 2
- GusX
- Features
- dicots
- Other markers
- Amp
- Size
- 4,984 bp, circular
- Record date
- 04-OCT-2017
Construct
- MCS · 11 unique sites · at 103, 165
- SfiI ×2 · flanks the MCS · at 118, 2,208
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 103 | between ColE1 and GusX |
| XbaI | T^CTAGA | 105 | between ColE1 and GusX |
| NcoI | C^CATGG | 113 | between ColE1 and GusX |
| NotI | GC^GGCCGC | 119 | between ColE1 and GusX |
| SpeI | A^CTAGT | 126 | between ColE1 and GusX |
| BamHI | G^GATCC | 132 | between ColE1 and GusX |
| SmaI (XmaI, TspMI) | CCC^GGG | 140 | between ColE1 and GusX |
| PstI | CTGCA^G | 148 | between ColE1 and GusX |
| EcoRI | G^AATTC | 150 | between ColE1 and GusX |
| HindIII | A^AGCTT | 156 | between ColE1 and GusX |
| MluI | A^CGCGT | 165 | between ColE1 and GusX |
| BbsI (BpiI) | GAAGAC(2/6) | 391 | in GusX |
| BstEII (Eco91I) | G^GTNACC | 451 | in GusX |
| HpaI (KspAI) | GTT^AAC | 478 | in GusX |
| BstBI (Bsp119I) | TT^CGAA | 522 | in GusX |
| EcoRV (Eco32I) | GAT^ATC | 739 | in GusX |
| AarI | CACCTGC(4/8) | 784 | in GusX |
| SalI | G^TCGAC | 1,994 | between GusX and OCS |
| BsmBI (Esp3I) | CGTCTC(1/5) | 2,014 | in OCS |
| StuI (Eco147I) | AGG^CCT | 2,202 | between OCS and Amp |
| AflII (BspTI) | C^TTAAG | 2,204 | between OCS and Amp |
| BglII | A^GATCT | 2,214 | between OCS and Amp |
| ApaI (PspOMI) | GGGCC^C | 2,225 | between OCS and Amp |
| KpnI (Acc65I) | GGTAC^C | 2,231 | between OCS and Amp |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 4,802 | between ColE1 and GusX |
Cut twice: SfiI (118, 2,208), EagI (119, 1,803), PvuI (2,383, 3,428), PvuII (2,352, 4,865), AatII (534, 1,566), XmnI (994, 3,197).
No site: SphI, XhoI, NdeI, NheI, ClaI, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, NsiI, BsrGI, SacII.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| GusX | Coding sequence CDS | 182 | 1,993 | 1,812 | + |
| OCS | Terminator misc_feature | 1,999 | 2,194 | 196 | + |
| Amp | Selection marker misc_feature | 3,010 | 3,870 | 861 | + |
| ColE1 | Origin / backbone CDS | 3,913 | 4,629 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.