pHSP-mGFPM-ocs E040
Ligation of B594p2x35s-mGFPM-ocs* into A212pHSP-DGA*
- Promoter 1
- HSP
- Gene 1
- GFP
- Features
- dicots and monocots
- Other markers
- Amp
- Size
- 4,510 bp, circular
- Record date
- 13-JUL-2020
Construct
- MCS · 8 unique sites · at 504, 568
- SfiI ×2 · flanks the MCS · at 92, 1,708
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 77 | between ColE1 and HSP |
| XbaI | T^CTAGA | 79 | between ColE1 and HSP |
| NcoI | C^CATGG | 87 | between ColE1 and HSP |
| NotI | GC^GGCCGC | 93 | between ColE1 and HSP |
| EagI (Eco52I) | C^GGCCG | 93 | between ColE1 and HSP |
| BsrGI (Bsp1407I) | T^GTACA | 253 | in HSP |
| NsiI (Mph1103I) | ATGCA^T | 428 | in HSP |
| BbsI (BpiI) | GAAGAC(2/6) | 504 | in HSP |
| SpeI | A^CTAGT | 531 | between HSP and GFP |
| BamHI | G^GATCC | 538 | between HSP and GFP |
| SmaI (XmaI, TspMI) | CCC^GGG | 546 | between HSP and GFP |
| PstI | CTGCA^G | 554 | between HSP and GFP |
| EcoRI | G^AATTC | 556 | between HSP and GFP |
| HindIII | A^AGCTT | 562 | between HSP and GFP |
| MluI | A^CGCGT | 568 | between HSP and GFP |
| MfeI (MunI) | C^AATTG | 1,070 | in ST-LS1 |
| ClaI (BspDI) | AT^CGAT | 1,156 | in GFP |
| SalI | G^TCGAC | 1,494 | between GFP and OCS |
| BsmBI (Esp3I) | CGTCTC(1/5) | 1,514 | in OCS |
| StuI (Eco147I) | AGG^CCT | 1,702 | between OCS and Amp |
| AflII (BspTI) | C^TTAAG | 1,704 | between OCS and Amp |
| BglII | A^GATCT | 1,714 | between OCS and Amp |
| ApaI (PspOMI) | GGGCC^C | 1,725 | between OCS and Amp |
| XmnI (PdmI) | GAANN^NNTTC | 2,697 | in Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 3,231 | in Amp |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 4,302 | between ColE1 and HSP |
Cut twice: KpnI (806, 1,731), SfiI (92, 1,708), BstBI (350, 925), PvuI (1,883, 2,928), PvuII (1,852, 4,365).
No site: SphI, XhoI, NdeI, NheI, EcoRV, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, AgeI, HpaI, SacII, AatII, BstEII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| HSP | Other misc_feature | 100 | 530 | 431 | + |
| GFP | Coding sequence CDS | 585 | 963 | 379 | + |
| ST-LS1 | Coding sequence CDS | 964 | 1,152 | 189 | + |
| GFP | Coding sequence CDS | 1,153 | 1,490 | 338 | + |
| OCS | Terminator misc_feature | 1,499 | 1,694 | 196 | + |
| Amp | Selection marker misc_feature | 2,510 | 3,370 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 3,413 | 4,129 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.