pLGFP-ocs B636
Ligation of B629p35s-LGFP* into pOCS-BM*
- Promoter 2
- MCS
- Gene 2
- Link-GFP
- Features
- monocots and dicots
- Other markers
- Amp
- Size
- 4,125 bp, circular
- Record date
- 22-JAN-2016
Construct
- MCS · 13 unique sites · at 77, 142
- SfiI ×2 · flanks the MCS · at 92, 1,323
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 77 | between ColE1 and link |
| XbaI | T^CTAGA | 79 | between ColE1 and link |
| NcoI | C^CATGG | 87 | between ColE1 and link |
| NotI | GC^GGCCGC | 93 | between ColE1 and link |
| EagI (Eco52I) | C^GGCCG | 93 | between ColE1 and link |
| SpeI | A^CTAGT | 100 | between ColE1 and link |
| BamHI | G^GATCC | 106 | between ColE1 and link |
| SmaI (XmaI, TspMI) | CCC^GGG | 114 | between ColE1 and link |
| PstI | CTGCA^G | 122 | between ColE1 and link |
| EcoRI | G^AATTC | 124 | between ColE1 and link |
| HindIII | A^AGCTT | 130 | between ColE1 and link |
| MluI | A^CGCGT | 136 | between ColE1 and link |
| BsrGI (Bsp1407I) | T^GTACA | 142 | between ColE1 and link |
| BstBI (Bsp119I) | TT^CGAA | 539 | in gfpm |
| MfeI (MunI) | C^AATTG | 684 | in int |
| ClaI (BspDI) | AT^CGAT | 770 | in gfpm |
| SalI | G^TCGAC | 1,109 | between gfpm and Tocs |
| BsmBI (Esp3I) | CGTCTC(1/5) | 1,129 | in Tocs |
| StuI (Eco147I) | AGG^CCT | 1,317 | between Tocs and Amp |
| AflII (BspTI) | C^TTAAG | 1,319 | between Tocs and Amp |
| BglII | A^GATCT | 1,329 | between Tocs and Amp |
| ApaI (PspOMI) | GGGCC^C | 1,340 | between Tocs and Amp |
| KpnI (Acc65I) | GGTAC^C | 1,346 | between Tocs and Amp |
| XmnI (PdmI) | GAANN^NNTTC | 2,312 | in Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 2,846 | in Amp |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 3,917 | between ColE1 and link |
Cut twice: SfiI (92, 1,323), PvuI (1,498, 2,543), PvuII (1,467, 3,980).
No site: SphI, XhoI, NdeI, NheI, EcoRV, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, AgeI, HpaI, NsiI, SacII, AatII, BstEII, BbsI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| link | Other misc_feature | 148 | 198 | 51 | + |
| gfpm | Coding sequence CDS | 199 | 577 | 379 | + |
| int | Site CDS | 578 | 766 | 189 | + |
| gfpm | Coding sequence CDS | 767 | 1,104 | 338 | + |
| Tocs | Terminator misc_feature | 1,114 | 1,309 | 196 | + |
| Amp | Selection marker misc_feature | 2,125 | 2,985 | 861 | + |
| ColE1 | Origin / backbone CDS | 3,028 | 3,744 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.