pLN35G-Flpi
Ligation of p9-35S-OCS* into pLNUG-FLPint*
- Promoter 2
- 35s
- Gene 2
- FLPint-GFPint
- Features
- FLP-GFP fusion
- Other markers
- Amp
- Size
- 6,097 bp, circular
- Record date
- 25-JUN-2010
Construct
- 35S-Pro–Flp–GFP · payload cassette, 140–2,966
- MCS · 4 unique sites · at 62, 127
- SfiI ×2 · flanks the 35S-Pro–Flp–GFP cassette · at 78, 3,281
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| ApaI (PspOMI) | GGGCC^C | 62 | between Amp and loxP |
| BglII | A^GATCT | 65 | between Amp and loxP |
| PacI | TTAAT^TAA | 88 | between Amp and loxP |
| NotI | GC^GGCCGC | 127 | between loxP and 35S-Pro |
| AgeI (BshTI) | A^CCGGT | 574 | between 35S-Pro and Flp |
| AatII (ZraI) | GACGT^C | 584 | between 35S-Pro and Flp |
| SphI (PaeI) | GCATG^C | 593 | in Flp |
| EcoRI | G^AATTC | 1,529 | in Flp |
| HindIII | A^AGCTT | 1,633 | in Flp |
| SpeI | A^CTAGT | 1,847 | in Flp |
| BamHI | G^GATCC | 2,050 | between Flp and GFP |
| AflII (BspTI) | C^TTAAG | 3,002 | in NOS |
| NcoI | C^CATGG | 3,279 | between LoxP and ColE1 |
| SacI (Ecl136II) | GAGCT^C | 3,297 | between LoxP and ColE1 |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 3,575 | between LoxP and ColE1 |
| BsaI (Eco31I) | GGTCTC(1/5) | 4,645 | in Amp |
Cut twice: XbaI (568, 3,287), NdeI (1,868, 2,046), ClaI (1,495, 2,632), EcoRV (468, 935), SfiI (78, 3,281), SwaI (1,199, 3,268), BstBI (970, 2,401), NsiI (1,868, 3,093), EagI (127, 2,055), PvuII (3,515, 6,028), XmnI (428, 5,183).
No site: SalI, PstI, SmaI, XhoI, NheI, MluI, AscI, SbfI, FseI, PmeI, AvrII, BsiWI, HpaI, StuI, BsrGI, SacII, BstEII, BsmBI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| loxP | Site misc_feature | 91 | 124 | 34 | + |
| 35S-Pro | Promoter misc_feature | 140 | 557 | 418 | + |
| Flp | Coding sequence CDS | 591 | 1,273 | 683 | + |
| int | Site CDS | 1,274 | 1,462 | 189 | + |
| Flp | Coding sequence CDS | 1,463 | 2,049 | 587 | + |
| GFP | Coding sequence CDS | 2,061 | 2,439 | 379 | + |
| int | Site CDS | 2,440 | 2,628 | 189 | + |
| GFP | Coding sequence CDS | 2,629 | 2,966 | 338 | + |
| NOS | Other misc_feature | 2,973 | 3,226 | 254 | + |
| LoxP | Site misc_feature | 3,233 | 3,266 | 34 | + |
| ColE1 | Origin / backbone misc_feature | 3,752 | 4,468 | 717 | − |
| Amp | Selection marker CDS | 4,511 | 5,371 | 861 | − |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.