pU10Tnos-U10Tocs C971
Ligation of NosU10-Spe-Mlu* into 204pUbiAtmu-ocs*
- Promoter 1
- Ubq10
- Promoter 2
- Ubq10
- Gene 2
- Ocs
- Features
- dicots
- Other markers
- Amp
- Size
- 6,394 bp, circular
- Record date
- 26-AUG-2020
Construct
- Pro-Ubq10At–(empty)–NOS-T · empty expression cassette, 126–1,882
- Pro-Ubq10At–(empty)–OCS · empty expression cassette, 1,903–3,604
- SfiI ×2 · flanks 2 cassettes · at 118, 3,618
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| NcoI | C^CATGG | 113 | between ColE1 and Pro-Ubq10At |
| NotI | GC^GGCCGC | 119 | between ColE1 and Pro-Ubq10At |
| EagI (Eco52I) | C^GGCCG | 119 | between ColE1 and Pro-Ubq10At |
| SpeI | A^CTAGT | 1,602 | between Pro-Ubq10At and NOS-T |
| BamHI | G^GATCC | 1,611 | between Pro-Ubq10At and NOS-T |
| NsiI (Mph1103I) | ATGCA^T | 1,749 | in NOS-T |
| SmaI (XmaI, TspMI) | CCC^GGG | 1,890 | between NOS-T and Pro-Ubq10At |
| PstI | CTGCA^G | 3,384 | between Pro-Ubq10At and OCS |
| HindIII | A^AGCTT | 3,389 | between Pro-Ubq10At and OCS |
| MluI | A^CGCGT | 3,398 | between Pro-Ubq10At and OCS |
| StuI (Eco147I) | AGG^CCT | 3,612 | between OCS and Amp |
| ApaI (PspOMI) | GGGCC^C | 3,635 | between OCS and Amp |
| KpnI (Acc65I) | GGTAC^C | 3,641 | between OCS and Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 5,141 | in Amp |
Cut twice: EcoRI (786, 2,563), SfiI (118, 3,618), AflII (1,658, 3,614), PvuII (3,762, 6,275), BbsI (1,406, 3,183), BsmBI (1,887, 3,424).
No site: SphI, XhoI, NdeI, NheI, ClaI, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, BstBI, HpaI, BsrGI, SacII, AatII, BstEII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| Pro-Ubq10At | Promoter misc_feature | 126 | 1,601 | 1,476 | + |
| TATA | Site misc_feature | 1,197 | 1,200 | 4 | + |
| Ubi cds 5 | Promoter misc_feature | 1,230 | 1,293 | 64 | + |
| íntron | Other misc_feature | 1,296 | 1,599 | 304 | + |
| NOS-T | Terminator misc_feature | 1,629 | 1,882 | 254 | + |
| Pro-Ubq10At | Promoter misc_feature | 1,903 | 3,378 | 1,476 | + |
| TATA | Site misc_feature | 2,974 | 2,977 | 4 | + |
| Ubi cds 5 | Promoter misc_feature | 3,007 | 3,070 | 64 | + |
| íntron | Other misc_feature | 3,073 | 3,376 | 304 | + |
| OCS | Terminator misc_feature | 3,409 | 3,604 | 196 | + |
| Amp | Selection marker misc_feature | 4,420 | 5,280 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 5,323 | 6,039 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.