pUbiAT-OCS
Ligation of pUbiAT-Gus-Or* into pOCS-BM*
- Promoter 2
- Ubq10
- Features
- Ubiq10At promoter from Arabidopsis
- Other markers
- Amp
- Size
- 4,634 bp, circular
- Record date
- 20-JUN-2013
Construct
- UbiAT-Pro–(empty)–OCS · empty expression cassette, 26–1,746
- MCS · 7 unique sites · at 1,504, 1,566
- SfiI ×2 · flanks the UbiAT-Pro–(empty)–OCS cassette · at 20, 1,760
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| NcoI | C^CATGG | 15 | between ColE1 and UbiAT-Pro |
| NotI | GC^GGCCGC | 21 | between ColE1 and UbiAT-Pro |
| EagI (Eco52I) | C^GGCCG | 21 | between ColE1 and UbiAT-Pro |
| BbsI (BpiI) | GAAGAC(2/6) | 1,308 | in intron |
| SpeI | A^CTAGT | 1,504 | between UbiAT-Pro and OCS |
| BamHI | G^GATCC | 1,510 | between UbiAT-Pro and OCS |
| SmaI (XmaI, TspMI) | CCC^GGG | 1,518 | between UbiAT-Pro and OCS |
| PstI | CTGCA^G | 1,526 | between UbiAT-Pro and OCS |
| HindIII | A^AGCTT | 1,534 | between UbiAT-Pro and OCS |
| MluI | A^CGCGT | 1,540 | between UbiAT-Pro and OCS |
| BsmBI (Esp3I) | CGTCTC(1/5) | 1,566 | in OCS |
| StuI (Eco147I) | AGG^CCT | 1,754 | between OCS and Amp |
| AflII (BspTI) | C^TTAAG | 1,756 | between OCS and Amp |
| ApaI (PspOMI) | GGGCC^C | 1,777 | between OCS and Amp |
| KpnI (Acc65I) | GGTAC^C | 1,783 | between OCS and Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 3,283 | in Amp |
Cut twice: EcoRI (688, 1,528), SalI (866, 1,546), SacI (5, 1,121), BglII (1,433, 1,766), EcoRV (1,331, 1,364), SfiI (20, 1,760), PvuII (1,904, 4,417), XmnI (289, 2,749), SapI (1,126, 4,354).
No site: SphI, XhoI, NdeI, NheI, ClaI, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, MfeI, AgeI, BstBI, HpaI, NsiI, BsrGI, SacII, AatII, BstEII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| UbiAT-Pro | Promoter misc_feature | 26 | 1,503 | 1,478 | + |
| TATA | Site misc_signal | 1,099 | 1,102 | 4 | + |
| Ubi cds 5` | Coding sequence CDS | 1,132 | 1,195 | 64 | + |
| intron | Site misc_feature | 1,198 | 1,501 | 304 | + |
| TATA | Site misc_signal | 1,263 | 1,266 | 4 | + |
| OCS | Terminator misc_feature | 1,551 | 1,746 | 196 | + |
| Amp | Selection marker misc_feature | 2,562 | 3,422 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 3,465 | 4,181 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.
Used in
- The Arabidopsis AAC Proteins CIL and CIA2 Are Sub-functionalized Paralogs Involved in Chloroplast Development – Li M, Ruwe H, Melzer M, et al., Frontiers in Plant Science 2021; 12:681375
- RNA-Guided Cas9-Induced Mutagenesis in Tobacco Followed by Efficient Genetic Fixation in Doubled Haploid Plants – Schedel S, Pencs S, Hensel G, et al., Frontiers in Plant Science 2017; 7
- A simple test for the cleavage activity of customized endonucleases in plants – Budhagatapalli N, Schedel S, Gurushidze M, et al., Plant Methods 2016; 12:18