pUbi-iF2
Ligation of Ubi-Promotor into p35iF2-2
- Promoter 2
- Ubi-maize
- Gene 2
- intron-fad2
- Features
- hairpin construct with intron, Ubi-maize RNAi constuct
- Other markers
- Amp
- Size
- 5,829 bp, circular
- Record date
- 13-JUL-2010
Construct
- P-Ubi-Zm–(empty)–Tocs · empty expression cassette, 34–2,941
- MCS · 4 unique sites · at 1,550, 1,572
- SfiI ×2 · flanks the P-Ubi-Zm–(empty)–Tocs cassette · at 20, 2,955
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SacI (Ecl136II) | GAGCT^C | 5 | between ColE1 and P-Ubi-Zm |
| NotI | GC^GGCCGC | 21 | between ColE1 and P-Ubi-Zm |
| EagI (Eco52I) | C^GGCCG | 21 | between ColE1 and P-Ubi-Zm |
| XhoI (PaeR7I) | C^TCGAG | 733 | in P-Ubi-Zm |
| NheI (BmtI) | G^CTAGC | 1,153 | in P-Ubi-Zm |
| ClaI (BspDI) | AT^CGAT | 1,266 | in P-Ubi-Zm |
| NsiI (Mph1103I) | ATGCA^T | 1,311 | in P-Ubi-Zm |
| BamHI | G^GATCC | 1,550 | between P-Ubi-Zm and sp-site |
| FseI | GGCCGG^CC | 1,562 | between P-Ubi-Zm and sp-site |
| SbfI (SdaI) | CCTGCA^GG | 1,569 | between P-Ubi-Zm and sp-site |
| EcoRI | G^AATTC | 1,572 | between P-Ubi-Zm and sp-site |
| HpaI (KspAI) | GTT^AAC | 2,394 | in iF2 |
| BbsI (BpiI) | GAAGAC(2/6) | 2,593 | in iF2 |
| SphI (PaeI) | GCATG^C | 2,626 | in iF2 |
| BsrGI (Bsp1407I) | T^GTACA | 2,721 | between sp-site and Tocs |
| HindIII | A^AGCTT | 2,728 | between sp-site and Tocs |
| MluI | A^CGCGT | 2,735 | between sp-site and Tocs |
| BsmBI (Esp3I) | CGTCTC(1/5) | 2,761 | in Tocs |
| StuI (Eco147I) | AGG^CCT | 2,949 | between Tocs and Amp |
| AflII (BspTI) | C^TTAAG | 2,951 | between Tocs and Amp |
| KpnI (Acc65I) | GGTAC^C | 2,978 | between Tocs and Amp |
| XmnI (PdmI) | GAANN^NNTTC | 3,944 | in Amp |
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 5,549 | between ColE1 and P-Ubi-Zm |
Cut twice: SalI (2,270, 2,741), NcoI (15, 1,080), SpeI (1,543, 2,445), ApaI (1,093, 2,972), SfiI (20, 2,955), MfeI (267, 2,714), PvuII (3,099, 5,612), BsaI (1,716, 4,478).
No site: SmaI, EcoRV, AscI, PacI, PmeI, SwaI, AvrII, BsiWI, AgeI, BstBI, SacII, AatII, BstEII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| P-Ubi-Zm | Promoter misc_feature | 34 | 1,542 | 1,509 | + |
| sp-site | Site misc_feature | 1,577 | 1,585 | 9 | + |
| iF2 | Other misc_feature | 1,580 | 2,711 | 1,132 | + |
| sp-site | Site misc_feature | 2,709 | 2,713 | 5 | + |
| Tocs | Terminator misc_feature | 2,746 | 2,941 | 196 | + |
| Amp | Selection marker CDS | 3,757 | 4,617 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 4,660 | 5,376 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.
Used in
- The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize – Zhou LZ, Wang L, Chen X, et al., The Plant Cell 2024; 36:1673–1696