pHC5-MBP-CBE-nC9 F349
Ligation of MBP_Assembly* into F348pHC5-CBE-nC9* corrected sequence
- Gene 2
- CBE
- Size
- 8,429 bp, circular
- Record date
- 08-DEC-2022
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SpeI | A^CTAGT | 69 | between O1 and MBP |
| NcoI | C^CATGG | 75 | between O1 and MBP |
| BsaI (Eco31I) | GGTCTC(1/5) | 203 | in MBP |
| BsiWI (Pfl23II) | C^GTACG | 430 | in MBP |
| BglII | A^GATCT | 499 | in MBP |
| SalI | G^TCGAC | 1,323 | between MBP and GamApo_String1 |
| SacII (Cfr42I) | CCGC^GG | 1,687 | in GAM |
| AatII (ZraI) | GACGT^C | 2,654 | in SGGS Linker' |
| BamHI | G^GATCC | 2,696 | between GamApo_String2 and nCas9 |
| BsmBI (Esp3I) | CGTCTC(1/5) | 2,891 | in nCas9 |
| SwaI (SmiI) | ATTT^AAAT | 3,219 | in nCas9 |
| NheI (BmtI) | G^CTAGC | 3,797 | in nCas9 |
| SphI (PaeI) | GCATG^C | 3,959 | in nCas9 |
| MluI | A^CGCGT | 5,270 | in nCas9 |
| XhoI (PaeR7I) | C^TCGAG | 6,806 | between nCas9 and O2 |
Cut twice: HindIII (3,705, 6,050), KpnI (1,896, 4,476), BstBI (1,854, 4,036), HpaI (2,110, 2,389), PvuI (465, 7,320), PvuII (2,524, 4,783), BstEII (1,442, 1,636).
No site: XbaI, SmaI, NotI, ClaI, ApaI, AscI, PacI, SfiI, SbfI, FseI, PmeI, AvrII, AgeI, StuI, NsiI, AflII, BsrGI, EagI, SapI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| O1 | Site CDS | 30 | 50 | 21 | + |
| MBP | Coding sequence CDS | 77 | 1,323 | 1,247 | + |
| 6xHis affinity tag | Site CDS | 89 | 106 | 18 | + |
| thrombin recognition and cleavage site | Site CDS | 116 | 133 | 18 | + |
| 'GamApo_String1 | Coding sequence CDS | 1,324 | 2,024 | 701 | + |
| TEV | Site misc_feature | 1,331 | 1,351 | 21 | + |
| GAM | Coding sequence CDS | 1,352 | 1,922 | 571 | + |
| APOBEC1 | Coding sequence CDS | 1,923 | 2,606 | 684 | + |
| GamApo_String2 | Coding sequence CDS | 1,995 | 2,696 | 702 | + |
| SGGS Linker' | Coding sequence CDS | 2,606 | 2,696 | 91 | + |
| nCas9 | Coding sequence CDS | 2,703 | 6,801 | 4,099 | + |
| D10A | Site misc_feature | 2,726 | 2,728 | 3 | + |
| O2 | Site CDS | 6,827 | 6,847 | 21 | + |
| ORF-1 | Coding sequence CDS | 6,902 | 7,762 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 7,765 | 8,429 | 665 | + |
| LC | Site misc_feature | 7,987 | 7,987 | 1 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.