p35s-Q4GFP C834
Ligation of m4GFP_BamEco* into p35-OCS-BM*
- Promoter 1
- 35s
- Gene 2
- EGFP
- Other markers
- Amp
- Size
- 6,593 bp, circular
- Record date
- 27-FEB-2019
Construct
- 35S-Pro–Q1–ORF-1–Q2–Q3–Q4 · OCS · payload cassette, 106–3,777
- SfiI ×2 · flanks the 35S-Pro–Q1–ORF-1–Q2–Q3–Q4 cassette · at 92, 3,791
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| XbaI | T^CTAGA | 79 | between ColE1 and 35S-Pro |
| NotI | GC^GGCCGC | 93 | between ColE1 and 35S-Pro |
| EagI (Eco52I) | C^GGCCG | 93 | between ColE1 and 35S-Pro |
| EcoRV (Eco32I) | GAT^ATC | 434 | in 35S-Pro |
| SpeI | A^CTAGT | 527 | in 35S-Pro |
| BamHI | G^GATCC | 534 | between 35S-Pro and Q1' |
| BsiWI (Pfl23II) | C^GTACG | 1,426 | in Q2' |
| NdeI | CA^TATG | 1,543 | in Q2' |
| SacII (Cfr42I) | CCGC^GG | 2,758 | in Q3 |
| ClaI (BspDI) | AT^CGAT | 3,226 | in Q4'' |
| EcoRI | G^AATTC | 3,559 | between ORF-1 and OCS |
| MluI | A^CGCGT | 3,571 | between ORF-1 and OCS |
| SalI | G^TCGAC | 3,577 | between ORF-1 and OCS |
| StuI (Eco147I) | AGG^CCT | 3,785 | between OCS and Amp |
| ApaI (PspOMI) | GGGCC^C | 3,808 | between OCS and Amp |
Cut twice: KpnI (2,300, 3,814), SfiI (92, 3,791), HpaI (2,567, 2,909), PvuI (3,966, 5,011), AatII (613, 2,143), XmnI (394, 4,780), BsaI (2,033, 5,314), SapI (2,103, 6,385).
No site: PstI, SphI, SmaI, NheI, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, MfeI, AgeI, BstBI, NsiI, BstEII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S-Pro | Promoter misc_feature | 106 | 528 | 423 | + |
| TATA | Site CDS | 494 | 500 | 7 | + |
| 'Q1' | Coding sequence CDS | 535 | 1,313 | 779 | + |
| ORF-1 | Coding sequence CDS | 549 | 3,559 | 3,011 | + |
| Linker | Site misc_feature | 1,263 | 1,313 | 51 | + |
| Q2' | Coding sequence CDS | 1,263 | 2,078 | 816 | + |
| Linker | Site misc_feature | 2,028 | 2,078 | 51 | + |
| Q3 | Coding sequence CDS | 2,048 | 2,822 | 775 | + |
| Linker | Site misc_feature | 2,793 | 2,843 | 51 | + |
| Q4'' | Coding sequence CDS | 2,793 | 3,559 | 767 | + |
| OCS | Terminator misc_feature | 3,582 | 3,777 | 196 | + |
| Amp | Selection marker misc_feature | 4,593 | 5,453 | 861 | + |
| ColE1 | Origin / backbone misc_feature | 5,496 | 6,212 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.