pET28-CBE-nC9s G803
E. coli expression vector with CBE.
GenBank (.gbk)SnapGene (.dna)FASTA.seq
Opens in SnapGene Viewer (free), Benchling, ApE and Geneious.
Restriction sites
| Unique cutter · site | Cuts at | Location |
|---|---|---|
| XbaIT^CTAGA | 66 | between lac\operator and His-Tag |
| NcoIC^CATGG | 105 | between lac\operator and His-Tag |
| SacII (Cfr42I)CCGC^GG | 472 | in GAM |
| AatII (ZraI)GACGT^C | 1,439 | in SGGS Linker' |
| BamHIG^GATCC | 1,481 | between SGGS Linker' and nCas9 |
| SwaI (SmiI)ATTT^AAAT | 2,004 | in nCas9 |
| NheI (BmtI)G^CTAGC | 2,582 | in nCas9 |
| SacI (Ecl136II)GAGCT^C | 5,443 | in nCas9 |
| XhoI (PaeR7I)C^TCGAG | 5,591 | between nCas9 and His-Tag |
| PvuICGAT^CG | 6,697 | in kan\sequence |
| SmaI (XmaI, TspMI)CCC^GGG | 6,821 | in kan\sequence |
| ClaI (BspDI)AT^CGAT | 7,002 | in kan\sequence |
| SapI (BspQI, LguI)GCTCTTC(1/4) | 8,010 | between ori and bom |
| ApaI (PspOMI)GGGCC^C | 9,788 | in lac\I |
| BglIIA^GATCT | 10,713 | between lac\I and T7 promoter |
Cut twice: HindIII (2,490, 4,835), SphI (2,744, 10,524), KpnI (681, 3,261), NdeI (1,962, 3,397), MluI (4,055, 9,991), BstBI (639, 2,821), NsiI (6,583, 6,849).
No site: SalI, NotI, SpeI, AscI, PacI, SfiI, SbfI, FseI, PmeI, AvrII, BsiWI, AgeI, StuI, AflII, BsrGI, EagI, BsaI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
- Gene 2
- CBE
- Other markers
- kan\sequence
- Size
- 10,713 bp, circular
- Record date
- 03-JUL-2025
Construct
- T7 promoter–GAM–APOBEC1–nCas9 · payload cassette, 20–5,590
Ligation of Nhe-Xho* into F611pet28-CBE-nC9*
Read out of the GenBank file; compare every vector in the multiple expression cassettes table.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| T7 promoter | Site CDS | 20 | 37 | 18 | + |
| lac\operator | Site CDS | 36 | 66 | 31 | + |
| His-Tag | Site CDS | 119 | 136 | 18 | + |
| 'TEV | Site misc_feature | 136 | 136 | 1 | + |
| GAM | Coding sequence CDS | 137 | 707 | 571 | + |
| APOBEC1 | Coding sequence CDS | 708 | 1,391 | 684 | + |
| SGGS Linker' | Coding sequence CDS | 1,391 | 1,481 | 91 | + |
| nCas9 | Coding sequence CDS | 1,487 | 5,590 | 4,104 | + |
| D10A | Site misc_feature | 1,511 | 1,513 | 3 | + |
| His-Tag | Site CDS | 5,597 | 5,614 | 18 | + |
| Stop | Site CDS | 5,615 | 5,617 | 3 | + |
| f1 ori | Origin / backbone CDS | 5,765 | 6,220 | 456 | + |
| kan\sequence | Selection marker CDS | 6,315 | 7,127 | 813 | − |
| ori | Origin / backbone CDS | 7,249 | 7,837 | 589 | + |
| bom | Origin / backbone CDS | 8,023 | 8,162 | 140 | + |
| rop | Coding sequence CDS | 8,264 | 8,455 | 192 | + |
| CAP binding site | Site CDS | 9,230 | 9,251 | 22 | + |
| lac\I | Coding sequence CDS | 9,267 | 10,346 | 1,080 | − |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.
How to cite
Please cite the vector like this in your Methods section.
pET28-CBE-nC9s (G803; DNA Cloning Service e.K., Hamburg, Germany; https://dna-cloning.com/vectors/puc/pET28-CBE-nC9s)Your paper uses one of our vectors? Tell us.