pUbiZmF-LGFP B634
Ligation of B629p35s-LGFP* into B537pUbiZmF-Tocs*
- Promoter 2
- UbiZm
- Gene 2
- Link-GFP
- Features
- monocots
- Other markers
- Amp
- Size
- 6,128 bp, circular
- Record date
- 08-FEB-2016
Construct
- UbiZm–gfpm · Tocs · payload cassette, 439–3,638
- MCS · 5 unique sites · at 2,435, 2,471
- SfiI ×2 · flanks the UbiZm–gfpm cassette · at 418, 3,652
Read out of the GenBank file; compare every vector in the multiple expression cassettes and cloning sites table.
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Restriction sites
| Unique cutter | Site | Cuts at | Location |
|---|---|---|---|
| SapI (BspQI, LguI) | GCTCTTC(1/4) | 118 | between ColE1 and UbiZm |
| SacI (Ecl136II) | GAGCT^C | 403 | between ColE1 and UbiZm |
| NotI | GC^GGCCGC | 419 | between ColE1 and UbiZm |
| EagI (Eco52I) | C^GGCCG | 419 | between ColE1 and UbiZm |
| SpeI | A^CTAGT | 426 | between ColE1 and UbiZm |
| XhoI (PaeR7I) | C^TCGAG | 1,134 | in UbiZm |
| NheI (BmtI) | G^CTAGC | 1,553 | in UbiZm |
| BamHI | G^GATCC | 2,435 | between UbiZm and link |
| SmaI (XmaI, TspMI) | CCC^GGG | 2,443 | between UbiZm and link |
| HindIII | A^AGCTT | 2,459 | between UbiZm and link |
| MluI | A^CGCGT | 2,465 | between UbiZm and link |
| BsrGI (Bsp1407I) | T^GTACA | 2,471 | between UbiZm and link |
| BstBI (Bsp119I) | TT^CGAA | 2,868 | in gfpm |
| BsmBI (Esp3I) | CGTCTC(1/5) | 3,458 | in Tocs |
| StuI (Eco147I) | AGG^CCT | 3,646 | between Tocs and Amp |
| AflII (BspTI) | C^TTAAG | 3,648 | between Tocs and Amp |
| KpnI (Acc65I) | GGTAC^C | 3,675 | between Tocs and Amp |
| XmnI (PdmI) | GAANN^NNTTC | 4,641 | in Amp |
| BsaI (Eco31I) | GGTCTC(1/5) | 5,175 | in Amp |
Cut twice: EcoRI (1,830, 2,453), SalI (1,034, 3,438), NcoI (413, 1,480), BglII (1,380, 3,658), ApaI (1,493, 3,669), SfiI (418, 3,652), MfeI (672, 3,013), NsiI (1,976, 2,201), PvuI (3,827, 4,872), PvuII (181, 3,796).
No site: SphI, EcoRV, AscI, PacI, SbfI, FseI, PmeI, SwaI, AvrII, BsiWI, AgeI, HpaI, SacII, AatII, BstEII, BbsI, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| UbiZm | Promoter misc_feature | 439 | 2,432 | 1,994 | + |
| link | Other misc_feature | 2,477 | 2,527 | 51 | + |
| gfpm | Coding sequence CDS | 2,528 | 2,906 | 379 | + |
| int | Site CDS | 2,907 | 3,095 | 189 | + |
| gfpm | Coding sequence CDS | 3,096 | 3,433 | 338 | + |
| Tocs | Terminator misc_feature | 3,443 | 3,638 | 196 | + |
| Amp | Selection marker misc_feature | 4,454 | 5,314 | 861 | + |
| ColE1 | Origin / backbone CDS | 5,357 | 6,073 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.