pet28-MBP-HisOpti F455
E. coli expression vector with MBP.
GenBank (.gbk)SnapGene (.dna)FASTA.seq
Opens in SnapGene Viewer (free), Benchling, ApE and Geneious.
Restriction sites
| Unique cutter · site | Cuts at | Location |
|---|---|---|
| XbaIT^CTAGA | 67 | between lacI and ORF-1 |
| BsaI (Eco31I)GGTCTC(1/5) | 228 | in malE (mutated) |
| BsiWI (Pfl23II)C^GTACG | 455 | in malE (mutated) |
| SalIG^TCGAC | 1,348 | between the two ORF-1 |
| SwaI (SmiI)ATTT^AAAT | 1,948 | in cas9 |
| NheI (BmtI)G^CTAGC | 2,526 | in cas9 |
| BstBI (Bsp119I)TT^CGAA | 2,765 | in cas9 |
| KpnI (Acc65I)GGTAC^C | 3,205 | in cas9 |
| EagI (Eco52I)C^GGCCG | 5,533 | in Spacer |
| PstICTGCA^G | 6,311 | in NLS |
| XhoI (PaeR7I)C^TCGAG | 6,348 | in ORF-1 |
| SmaI (XmaI, TspMI)CCC^GGG | 7,579 | in aph(3')-Ia |
| ClaI (BspDI)AT^CGAT | 7,760 | in aph(3')-Ia |
| SapI (BspQI, LguI)GCTCTTC(1/4) | 8,768 | between aph(3')-Ia and bom |
| ApaI (PspOMI)GGGCC^C | 10,549 | in lacI |
Cut twice: EcoRI (1,335, 2,767), BamHI (1,329, 4,805), HindIII (2,434, 4,779), BglII (1, 524), NsiI (7,341, 7,607), BstEII (5,768, 10,570).
No site: NotI, SpeI, AscI, PacI, SfiI, SbfI, FseI, PmeI, AvrII, AgeI, StuI, AflII, BsrGI, SacII, AatII, AarI.
Computed from the GenBank sequence for 50 common enzymes; positions are the cut in the top strand, without Dam/Dcm methylation. Compare vectors in the restriction site finder.
- Gene 2
- MBP
- Size
- 11,473 bp, circular
- Record date
- 26-APR-2023
Ligation of his-tag MBP 2* into C704pet28MBP-Cas9C-R9*
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| ORF-1 | Coding sequence CDS | 137 | 1,348 | 1,212 | + |
| ORF-1' | Coding sequence CDS | 137 | 1,348 | 1,212 | + |
| thrombin recognition and cleavage site | Site CDS | 141 | 158 | 18 | + |
| malE (mutated) | Coding sequence CDS | 165 | 1,265 | 1,101 | + |
| ORF-1 | Coding sequence CDS | 1,349 | 6,374 | 5,026 | + |
| TEV | Site misc_feature | 1,356 | 1,376 | 21 | + |
| R9 | Site misc_feature | 1,377 | 1,406 | 30 | + |
| linker | Site misc_feature | 1,407 | 1,427 | 21 | + |
| cas9 | Coding sequence CDS | 1,428 | 5,531 | 4,104 | + |
| D10A | Site misc_feature | 1,455 | 1,457 | 3 | + |
| Spacer | Coding sequence CDS | 5,532 | 5,588 | 57 | + |
| 'HA | Site misc_feature | 5,591 | 5,591 | 1 | + |
| GFP' | Other misc_feature | 5,592 | 6,299 | 708 | + |
| NLS | Site misc_feature | 6,300 | 6,347 | 48 | + |
| 6xHis affinity tag | Site CDS | 6,354 | 6,371 | 18 | + |
| aph(3')-Ia | Coding sequence CDS | 7,070 | 7,885 | 816 | − |
| bom | Origin / backbone misc_feature | 8,781 | 8,923 | 143 | − |
| rop | Coding sequence CDS | 9,025 | 9,216 | 192 | − |
| lacI | Coding sequence CDS | 10,025 | 11,107 | 1,083 | − |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.
How to cite
Please cite the vector like this in your Methods section.
pet28-MBP-HisOpti (F455; DNA Cloning Service e.K., Hamburg, Germany; https://dna-cloning.com/vectors/puc/pet28-MBP-HisOpti)Your paper uses one of our vectors? Tell us.