| Price list | ||||
| We can do the cloning in any vector you want. | ||||
| Nr. | A. Vectors and Bacteria | Euro | ||
| 1000 | Vectors from the website | 250,00 € | ||
| 1001 | Special vectors from the website | 300,00 € | ||
| 1002 | Vectors adapted to customers | on request | ||
| 1003 | RNAi or recombinase vectors | 250,00 € | ||
| 1004 | Agrobacterium strain GV3101-pMP90RK | 200,00 € | ||
| 1005 | Cas9 vectors | 400,00 € | ||
| 1006 | DCS blue supercompetent cells, Transformation rate 1x109/µg DNA | 48X50 µl single shot | 192,00 € | |
| B. Cloning from plasmids | ||||
| 2010 | Cloning of a DNA fragment into a vector of your choice. | (sequencing not included) | 250,00 € | |
| 2020 | Cloning of a second DNA fragment into the same vector | (sequencing not included) | 200,00 € | |
| 2021 | Cloning of a third DNA fragment into the same vector | (sequencing not included) | 175,00 € | |
| 2030 | or cloning of the same DNA fragment into another vector | (sequencing not included) | 200,00 € | |
| 2040 | or cloning of a DNA fragment into a predigested vector | (sequencing not included) | 200,00 € | |
| 2050 | Cloning of a DNA fragment into a vector with the same selection marker | (sequencing not included) | 300,00 € | |
| 2051 | Cloning of a second DNA fragment into a vector with the same selection marker | (sequencing not included) | 250,00 € | |
| 2052 | Cloning of a third DNA fragment into a vector with the same selection marker | (sequencing not included) | 225,00 € | |
| 2060 | Seamless cloning of a DNA fragment into a vector of your choice. | (sequencing not included) | 350,00 € | |
| C. Cloning of amplified DNA fragments (from plasmids or genomic DNA) up to 5 kb | ||||
| 2110 | Cloning of a amplified DNA fragment into a vector of your choice. | (sequencing and primers not included) | 350,00 € | |
| 2111 | Cloning of a second amplified DNA fragment into the same vector | (sequencing and primers not included) | 300,00 € | |
| 2112 | Cloning of three amplified DNA fragments into the same vector | (sequencing and primers not included) | 275,00 € | |
| 2120 | or cloning of the same amplified DNA fragment into another vector | (sequencing and primers not included) | 300,00 € | |
| 2121 | or cloning of the same amplified DNA fragment into two other vectors | (sequencing and primers not included) | 275,00 € | |
| 2140 | Cloning of amplified DNA into a predigested vector | (sequencing not included) | 300,00 € | |
| 2160 | Cloning of amplified DNA into a TA cloning vector | 1 sample | 250,00 € | |
| 2161 | Cloning of amplified DNA into a TA cloning vector | 2-3 samples | 200,00 € | |
| 2162 | Cloning of amplified DNA into a TA cloning vector | 4-9 samples | 175,00 € | |
| 2170 | PCR-Cloning of DNA fragments larger 3.500 bp (1,00 € per 100 bp) | (sequencing and primers not included) | 350,00 € | |
| 2180 | Cloning of DNA into a pUC cloning vector | (sequencing not included) | 300,00 € | |
| D. Site directed mutagenesis and DNA assembling, Gene Synthesis | ||||
| 2200 | Site directed mutagenesis from plasmids (1 point mutation) | (sequencing and primers not included) | 300,00 € | |
| 2205 | Site directed mutagenesis from plasmids (1 complex mutation) | (sequencing and primers not included) | 350,00 € | |
| 2210 | Site directed mutagenesis from plasmids ( a second clone) | (sequencing and primers not included) | 250,00 € | |
| 2220 | Site directed mutagenesis from plasmids (2 point mutations) | (sequencing and primers not included) | 450,00 € | |
| 2230 | Site directed mutagenesis from plasmids (3 point mutations) | (sequencing and primers not included) | 550,00 € | |
| 2240 | Site directed mutagenesis from plasmids (4 point mutations) | (sequencing and primers not included) | 650,00 € | |
| 2300 | Assembling of 2 DNA fragments and cloning into a vector of your choice | (sequencing and primers not included) | 460,00 € | |
| 2301 | Assembling of 3 DNA fragments and cloning into a vector of your choice | (sequencing and primers not included) | 570,00 € | |
| 2302 | Assembling of 4 DNA fragments and cloning into a vector of your choice | (sequencing and primers not included) | 680,00 € | |
| 2303 | Assembling of 5 DNA fragments and cloning into a vector of your choice | (sequencing and primers not included) | 790,00 € | |
| 2310 | Assembling of 2 DNA fragments | (sequencing and primers not included) | 110,00 € | |
| 2400 | Cloning of a amplified DNA fragment into a specific amplified vector | (sequencing and primers not included) | 450,00 € | |
| 2500 | Cloning of a DNA fragment into a vector with specific restriction enzymes | 350,00 € | ||
| 2600 | Cloning of 1 target site into a Cas vector | (sequencing and primers included) | 350,00 € | |
| 2601 | Cloning of a second target site into a Cas vector | (sequencing and primers included) | 300,00 € | |
| 2602 | Cloning of a third target site into a Cas vector | (sequencing and primers included) | 275,00 € | |
| 2610 | Cloning of 1 double target site into a Cas vector | (sequencing and primers included) | 400,00 € | |
| 2611 | Cloning of 2 double target sites into a Cas vector | (sequencing and primers included) | 350,00 € | |
| 2612 | Cloning of 4 double target sites into a Cas vector | (sequencing and primers included) | 325,00 € | |
| 2613 | Cloning of 8 double target sites into a Cas vector | (sequencing and primers included) | 300,00 € | |
| 2700 | Cloning of 1 fourfold target sites into a Cas vector | (sequencing and primers included) | 1.200,00 € | |
| 2800 | Cloning of 1 sixfold target sites into a Cas vector | (sequencing and primers included) | 2.000,00 € | |
| 2810 | Cloning of 1 eightfold target sites into a Cas vector | (sequencing and primers included) | 2.800,00 € | |
| 2900 | Cloning of RVD into binary Talen construct | 50,00 € | ||
| E. Additional costs for cloning: If neccessary | ||||
| 3050 | Codon optimization for high expression | 0,10 € | ||
| 3050 | Codon optimization for optimum expression | 0,15 € | ||
| 3060 | Elongation of PCR product | 50,00 € | ||
| 3090 | Selection of bacteria with uncommon selection marker | hygromycine | 50,00 € | |
| 3090 | Cloning into E. coli strains other than XL1 | 50,00 € | ||
| 3105 | Cloning of plasmids with missing sequence | 50,00 € | ||
| 3110 | Cloning into low-copy plasmids additionally | 50,00 € | ||
| 3125 | Cloning of DNA fragments larger 2.5 kb | 50,00 € | ||
| 3126 | Cloning of DNA fragments larger 5 kb | 100,00 € | ||
| 3127 | Cloning of DNA fragments larger 7.5 kb | 150,00 € | ||
| 3128 | Cloning of DNA fragments larger 10 kb | 200,00 € | ||
| 3130 | Blunt-end cloning one site | 50,00 € | ||
| 3145 | Cloning into a single restriction site without blue-white selection. | 50,00 € | ||
| 3150 | Cloning of a DNA fragment smaller 100 bp | 25,00 € | ||
| 3160 | Cloning into vectors larger 10 kb | 25,00 € | ||
| 3170 | In frame cloning | 50,00 € | ||
| 3180 | Blunting of a restriction site | 50,00 € | ||
| 3190 | Restriction of DNA fragments with uncommon or expensive enzymes like: AgeI, AvrII, PciI, SphI, XmaI, ClaI- each site | costs of the Enzymes | ||
| 3195 | Isolation of a second clone | 25,00 € | ||
| 3196 | Correction of electronic sequences | each corrected bp | 5,00 € | |
| Primer | ||||
| 3210 | Primer for DNA amplification and sequencing per base | 0,50 € | ||
| 3220 | HPLC-purified primer per base (recomended for primers >30 bp) | 1,00 € | ||
| 3230 | Phosphorylated primer per base | 1,00 € | ||
| 3240 | Primer design | 12,00 € | ||
| 3241 | Primer design and synthesis for sequencing | 20,00 € | ||
| F. Additional service | ||||
| Cloning | ||||
| 4000 | Preparation of plasmid DNA for cloning | 50,00 € | ||
| 4100 | Genesynthesis with primers per base and cloning into a basic pUC vector | for genes smaller 625 bp | 250,00 € | |
| 4110 | Genesynthesis with primers per base and cloning into a basic pUC vector | for genes larger 625 bp | 0,35 € | |
| 4150 | cDNA synthesis from mRNA | 500,00 € | ||
| 4160 | 5´Race | sequencing included | 700,00 € | |
| 4170 | 3´Race | sequencing included | 500,00 € | |
| 4210 | Amplification and cloning of unknown genes with degenerated primers | on request | ||
| 4310 | Promotor isolation from cDNA-sequences -Isolation of genomic sequences with inverse PCR- | 1 sample | 1.500,00 € | |
| 4311 | Promotor isolation from cDNA-sequences -Isolation of genomic sequences with inverse PCR- | 2 samples | 1.000,00 € | |
| 4410 | Preparation of a cloning plan | each cloning step | 50,00 € | |
| 4510 | Documentation | 50,00 € | ||
| 4511 | Certification | 100,00 € | ||
| 4512 | Certification gene synthesis with primers | 200,00 € | ||
| 4512 | Preparation of electronic files | due to changed sequences | 100,00 € | |
| Transformation/Amplification | ||||
| 4600 | Transformation of DNA into E. coli | 1 sample | 25,00 € | |
| 4601 | Transformation of DNA into E. coli | 2-9 samples | 20,00 € | |
| 4602 | Transformation of DNA into E. coli | >10 samples | 15,00 € | |
| 4610 | Streaking bacteria on a agar plate for antibiotic selection | 1-10 samples | 10,00 € | |
| 4611 | Streaking bacteria on a agar plate for antibiotic selection | >10 samples | 5,00 € | |
| 4630 | Transformation of DNA into Agrobacterium with sequencing of retransformed E.coli | 1 sample | 150,00 € | |
| 4631 | Transformation of DNA into Agrobacterium with sequencing of retransformed E.coli | 2 samples | 100,00 € | |
| 4710 | Amplification of DNA with primers | 1 sample | 20,00 € | |
| 4711 | Amplification of DNA with primers | >10 samples | 10,00 € | |
| 4720 | High quality amplification from genomic DNA | 1 sample | 100,00 € | |
| DNA/RNA isolation | ||||
| 4750 | Isolation of genomic DNA from tissue | 1 sample | 400,00 € | |
| 4800 | Isolation of DNA/RNA from cells-Mini | 1 sample | 100,00 € | |
| 4801 | Isolation of DNA/RNA from cells-Maxi | 1 sample | 200,00 € | |
| 4802 | Isolation of DNA/RNA from cells-Mini | 100 samples | 20,00 € | |
| 4803 | Pesteling of probes | 100 samples | 15,00 € | |
| 4804 | RNA extraction | 100 samples | 10,00 € | |
| 4805 | Probe preparation for extraction | 100 samples | 5,00 € | |
| 4820 | Agarose gel purification | 1 sample | 50,00 € | |
| 4821 | Agarose gel purification | >10 samples | 20,00 € | |
| 4830 | Spin column purification | 1 sample | 10,00 € | |
| 4840 | Restriction digest | 1 sample | 20,00 € | |
| 4845 | UV measurement at 260 nm and DNA adjustment | 1 sample | 10,00 € | |
| 4850 | UV measurement at 260 nm | 1 sample | 5,00 € | |
| 4855 | Adjustment of DNA to 5 ng/µl | 1 sample | 100,00 € | |
| 4870 | Dilution of DNA to 1x106 copys | each sample | 100,00 € | |
| 4880 | Filling in tubes | each sample | 20,00 € | |
| 4890 | Storage of the DNA | each sample | 100,00 € | |
| 4860 | Agarose gel analysis | 1 sample | 10,00 € | |
| 4880 | Removal of genomic E. coli DNA from Maxiprep | 1 sample | 200,00 € | |
| 4900 | E. coli Miniprep | 1 sample | 20,00 € | |
| 4901 | E. coli Miniprep | 2-10 samples | 15,00 € | |
| 4902 | E. coli Miniprep | >10 samples | 10,00 € | |
| 4905 | Colony PCR | >10 samples | 5,00 € | |
| 4910 | Restriction and religation for Maxiprep | 1 sample | 100,00 € | |
| 4920 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 1 sample | 140,00 € |
| 4921 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 2 samples | 130,00 € |
| 4922 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 3 samples | 125,00 € |
| 4923 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 4 samples | 120,00 € |
| 4924 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 6 samples | 115,00 € |
| 4925 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 8 samples | 110,00 € |
| 4926 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 12 samples | 105,00 € |
| 4927 | Isolation of Maxi-Prep DNA (up to 500 µg) | high copy | 16 samples | 100,00 € |
| 4930 | Isolation of Mega-Prep DNA (up to 2.000 µg) | 1 sample | 400,00 € | |
| 4931 | Isolation of Mega-Prep DNA (up to 2.000 µg) | 2 samples | 350,00 € | |
| 4940 | Isolation of Giga-Prep DNA (up to 10.000 µg) | 1 sample | 1.500,00 € | |
| 4950 | Isolation of 60mg DNA | 1 sample | 4.000,00 € | |
| 4960 | Isolation of Maxi-Prep DNA (up to 200 µg) | low copy | 1 sample | 195,00 € |
| 4961 | Isolation of Maxi-Prep DNA (up to 200 µg) | low copy | 2 samples | 180,00 € |
| 4962 | Isolation of Maxi-Prep DNA (up to 200 µg) | low copy | 4 samples | 165,00 € |
| 4963 | Isolation of Maxi-Prep DNA (up to 200 µg) | low copy | 8 samples | 150,00 € |
| 4970 | Isolation of Maxi-Prep DNA (up to 500 µg) | Animal component free | 1 sample | 250,00 € |
| 4971 | Isolation of Maxi-Prep DNA (up to 500 µg) | Animal component free | 2 sample | 220,00 € |
| 4972 | Isolation of Maxi-Prep DNA (up to 500 µg) | Animal component free | 3 sample | 200,00 € |
| G. Sequencing price per sample (average reading length: 20-1000bp/99%) | ||||
| 5105 | Sequencing primer | 1 primer | 20,00 € | |
| 5106 | Primer for Gene synthesis (30 bp) | 1 primer | 30,00 € | |
| 5110 | Column DNA purification and sequencing | 100 samples | 6,50 € | |
| 5120 | Sequencing: normal | 11-100 samples | 9,00 € | |
| 5130 | Sequencing: normal | 101-1000 samples | 8,00 € | |
| 5410 | Sequencing and comparison with a known sequence or storage of the sequence | each sample | 20,00 € | |
| 5415 | Comparison with a known sequence | each sample | 5,00 € | |
| 5421 | Full documentation of the DNA in gene bank format | 50,00 € | ||
| 5422 | Sequencing of unknown DNA and documentation | 1000 bp | 100,00 € | |
| 5430 | Import of sequences from NCBI | 10,00 € | ||
| 5510 | DNA isolation, amplification and sequencing of genes from genomic DNA (16S-RNA, rubisco etc.) | 200,00 € | ||
| H. Supercompetent cells from your E. coli strain | ||||
| 6110 | Preparation of supercompetent cells from your E. coli strain in 2 ml vials (Transformation rate in 2ml vials: 1x109) | 128 vials | 384,00 € | |
| 6112 | Preparation of supercompetent cells from your E. coli strain in 2 ml vials (Transformation rate in 2ml vials: 1x109) | 64 vials | 192,00 € | |
| I. Plant transformation | ||||
| 7001 | Transformation into Arabidopsis | 500,00 € | ||
| 7002 | Transformation into Arabidopsis (T1 seeds tested) | 750,00 € | ||
| 7003 | Transformation into Arabidopsis (T2 seeds) | 1.250,00 € | ||
| 8000 | mRNA-synthesis with capping and Tailing from Plasmid, 50 µg | 450,00 € | ||
| 8001 | mRNA-synthesis with capping and Tailing from Plasmid, 100 µg | 1.000,00 € | ||
| 8002 | mRNA-synthesis with capping and Tailing from Plasmid, 150 µg | 1.200,00 € | ||
| 8003 | mRNA-synthesis with capping and Tailing from Plasmid, 200 µg | 1.400,00 € | ||
| 8004 | mRNA-synthesis with capping and Tailing from Plasmid, 300 µg | 1.800,00 € | ||
| 8005 | mRNA-synthesis with capping and Tailing from Plasmid, 400 µg | 2.200,00 € | ||
| 8006 | mRNA-synthesis with capping and Tailing from Plasmid, 600 µg | 3.000,00 € | ||
| Setup costs: | ||||
| When amplification failed: | costs for primer | |||
| When cloning failed: Due to our experience 99% of our cloning experiments are succesful | 50 % of cloning costs | |||
| miscellaneous | ||||
| 10001 | Delivery on ice | 30,00 € | ||
| 10001 | Delivery on dry ice | 20,00 € | ||
| 10002 | Delivery with FedEx | 50-100 € | ||
| 10003 | Delivery with Expeditors | 150,00 € | ||
| 10004 | payment with cheque | 10,00 € | ||
| 10005 | payment with PayPal in Euro | 5% | ||
| 10006 | PayPal payment in other currencies | 10% | ||
| 10006 | payment with wire transfer | free | ||
| 10007 | Export handling | 50,00 € | ||
| 10008 | Special Invoice Handling | 50,00 € | ||
| 10009 | Additional Cost estimation | 50,00 € | ||
| General recommendations | ||||
| Prices are without 19% VAT | ||||
| If you send us plasmid DNA for cloning, we prefer it purified by a midi/maxi prep in TE-buffer | ||||
| We need a minimum of 2µg vector DNA at a minimum concentration of 200ng/µl | ||||
| Other plasmids should be already transformed in E. coli on a agar plate. | ||||
| As a host strain we prefer DH5-alpha, XL1-blue or JM109 | ||||
| Generel cloning conditions will be valid for restriction enzymes with an overhang of | ||||
| at least 3 bases, DNA fragments up to 2.000 bp and high-copy plasmids. | ||||
| We don´t take any guaranty for genomic sequences | ||||
| DNA data should be readable by a computer | ||||
