Price list



We can do the cloning in any vector you want.







Nr. A. Vectors and Bacteria

Euro
1000 Vectors from the website

250,00 €
1001 Special vectors from the website

300,00 €
1002 Vectors adapted to customers

on request
1003 RNAi or recombinase vectors

250,00 €
1004 Agrobacterium strain GV3101-pMP90RK

200,00 €
1005 Cas9 vectors

400,00 €
1006 DCS blue supercompetent cells, Transformation rate 1x109/µg DNA
48X50 µl single shot 192,00 €






B. Cloning from plasmids


2010 Cloning of a DNA fragment into a vector of your choice.
(sequencing not included) 250,00 €
2020 Cloning of a second DNA fragment into the same vector
(sequencing not included) 200,00 €
2021 Cloning of a third DNA fragment into the same vector
(sequencing not included) 175,00 €
2030 or cloning of the same DNA fragment into another vector
(sequencing not included) 200,00 €
2040 or cloning of a DNA fragment into a predigested vector
(sequencing not included) 200,00 €
2050 Cloning of a DNA fragment into a vector with the same selection marker
(sequencing not included) 300,00 €
2051 Cloning of a second DNA fragment into a vector with the same selection marker
(sequencing not included) 250,00 €
2052 Cloning of a third DNA fragment into a vector with the same selection marker
(sequencing not included) 225,00 €
2060 Seamless cloning of a DNA fragment into a vector of your choice.
(sequencing not included) 350,00 €






C. Cloning of amplified DNA fragments (from plasmids or genomic DNA) up to 5 kb


2110 Cloning of a amplified DNA fragment into a vector of your choice.
(sequencing and primers not included) 350,00 €
2111 Cloning of a second amplified DNA fragment into the same vector
(sequencing and primers not included) 300,00 €
2112 Cloning of three amplified DNA fragments into the same vector
(sequencing and primers not included) 275,00 €
2120 or cloning of the same amplified DNA fragment into another vector
(sequencing and primers not included) 300,00 €
2121 or cloning of the same amplified DNA fragment into two other vectors
(sequencing and primers not included) 275,00 €
2140 Cloning of amplified DNA into a predigested vector
(sequencing not included) 300,00 €
2160 Cloning of amplified DNA into a TA cloning vector
1 sample 250,00 €
2161 Cloning of amplified DNA into a TA cloning vector
2-3 samples 200,00 €
2162 Cloning of amplified DNA into a TA cloning vector
4-9 samples 175,00 €
2170 PCR-Cloning of DNA fragments larger 3.500 bp (1,00 € per 100 bp)
(sequencing and primers not included) 350,00 €
2180 Cloning of DNA into a pUC cloning vector
(sequencing not included) 300,00 €






D. Site directed mutagenesis and DNA assembling, Gene Synthesis


2200 Site directed mutagenesis from plasmids (1 point mutation)
(sequencing and primers not included) 300,00 €
2205 Site directed mutagenesis from plasmids (1 complex mutation)
(sequencing and primers not included) 350,00 €
2210 Site directed mutagenesis from plasmids ( a second clone)
(sequencing and primers not included) 250,00 €
2220 Site directed mutagenesis from plasmids (2 point mutations)
(sequencing and primers not included) 450,00 €
2230 Site directed mutagenesis from plasmids (3 point mutations)
(sequencing and primers not included) 550,00 €
2240 Site directed mutagenesis from plasmids (4 point mutations)
(sequencing and primers not included) 650,00 €
2300 Assembling of 2 DNA fragments and cloning into a vector of your choice
(sequencing and primers not included) 460,00 €
2301 Assembling of 3 DNA fragments and cloning into a vector of your choice
(sequencing and primers not included) 570,00 €
2302 Assembling of 4 DNA fragments and cloning into a vector of your choice
(sequencing and primers not included) 680,00 €
2303 Assembling of 5 DNA fragments and cloning into a vector of your choice
(sequencing and primers not included) 790,00 €
2310 Assembling of 2 DNA fragments
(sequencing and primers not included) 110,00 €
2400 Cloning of a amplified DNA fragment into a specific amplified vector
(sequencing and primers not included) 450,00 €
2500 Cloning of a DNA fragment into a vector with specific restriction enzymes

350,00 €
2600 Cloning of 1 target site into a Cas vector
(sequencing and primers included) 350,00 €
2601 Cloning of a second target site into a Cas vector
(sequencing and primers included) 300,00 €
2602 Cloning of a third target site into a Cas vector
(sequencing and primers included) 275,00 €
2610 Cloning of 1 double target site into a Cas vector
(sequencing and primers included) 400,00 €
2611 Cloning of 2 double target sites into a Cas vector
(sequencing and primers included) 350,00 €
2612 Cloning of 4 double target sites into a Cas vector
(sequencing and primers included) 325,00 €
2613 Cloning of 8 double target sites into a Cas vector
(sequencing and primers included) 300,00 €
2700 Cloning of 1 fourfold target sites into a Cas vector
(sequencing and primers included) 1.200,00 €
2800 Cloning of 1 sixfold target sites into a Cas vector
(sequencing and primers included) 2.000,00 €
2810 Cloning of 1 eightfold target sites into a Cas vector
(sequencing and primers included) 2.800,00 €
2900 Cloning of RVD into binary Talen construct

50,00 €






E. Additional costs for cloning: If neccessary


3050 Codon optimization for high expression

0,10 €
3050 Codon optimization for optimum expression

0,15 €
3060 Elongation of PCR product

50,00 €
3090 Selection of bacteria with uncommon selection marker
hygromycine 50,00 €
3090 Cloning into E. coli strains other than XL1

50,00 €
3105 Cloning of plasmids with missing sequence

50,00 €
3110 Cloning into low-copy plasmids additionally

50,00 €
3125 Cloning of DNA fragments larger 2.5 kb

50,00 €
3126 Cloning of DNA fragments larger 5 kb

100,00 €
3127 Cloning of DNA fragments larger 7.5 kb

150,00 €
3128 Cloning of DNA fragments larger 10 kb

200,00 €
3130 Blunt-end cloning one site

50,00 €
3145 Cloning into a single restriction site without blue-white selection.

50,00 €
3150 Cloning of a DNA fragment smaller 100 bp

25,00 €
3160 Cloning into vectors larger 10 kb

25,00 €
3170 In frame cloning

50,00 €
3180 Blunting of a restriction site

50,00 €
3190
Restriction of DNA fragments with uncommon or expensive enzymes like:
AgeI, AvrII, PciI, SphI, XmaI, ClaI- each site

costs of the Enzymes
3195 Isolation of a second clone

25,00 €
3196 Correction of electronic sequences
each corrected bp 5,00 €











Primer


3210 Primer for DNA amplification and sequencing per base

0,50 €
3220 HPLC-purified primer per base (recomended for primers >30 bp)

1,00 €
3230 Phosphorylated primer per base

1,00 €
3240 Primer design

12,00 €
3241 Primer design and synthesis for sequencing

20,00 €






F. Additional service








Cloning


4000 Preparation of plasmid DNA for cloning

50,00 €
4100 Genesynthesis with primers per base and cloning into a basic pUC vector
for genes smaller 625 bp 250,00 €
4110 Genesynthesis with primers per base and cloning into a basic pUC vector
for genes larger 625 bp 0,35 €
4150 cDNA synthesis from mRNA

500,00 €
4160 5´Race
sequencing included 700,00 €
4170 3´Race
sequencing included 500,00 €
4210 Amplification and cloning of unknown genes with degenerated primers

on request
4310 Promotor isolation from cDNA-sequences -Isolation of genomic sequences with inverse PCR-
1 sample 1.500,00 €
4311 Promotor isolation from cDNA-sequences -Isolation of genomic
sequences with inverse PCR-

2 samples 1.000,00 €
4410 Preparation of a cloning plan
each cloning step 50,00 €
4510 Documentation

50,00 €
4511 Certification

100,00 €
4512 Certification gene synthesis with primers

200,00 €





4512 Preparation of electronic files
due to changed sequences 100,00 €






Transformation/Amplification


4600 Transformation of DNA into E. coli
1 sample 25,00 €
4601 Transformation of DNA into E. coli
2-9 samples 20,00 €
4602 Transformation of DNA into E. coli
>10 samples 15,00 €
4610 Streaking bacteria on a agar plate for antibiotic selection
1-10 samples 10,00 €
4611 Streaking bacteria on a agar plate for antibiotic selection
>10 samples 5,00 €
4630 Transformation of DNA into Agrobacterium with sequencing of retransformed E.coli
1 sample 150,00 €
4631 Transformation of DNA into Agrobacterium with sequencing of retransformed E.coli
2 samples 100,00 €
4710 Amplification of DNA with primers
1 sample 20,00 €
4711 Amplification of DNA with primers
>10 samples 10,00 €
4720 High quality amplification from genomic DNA
1 sample 100,00 €






DNA/RNA isolation


4750 Isolation of genomic DNA from tissue
1 sample 400,00 €
4800 Isolation of DNA/RNA from cells-Mini
1 sample 100,00 €
4801 Isolation of DNA/RNA from cells-Maxi
1 sample 200,00 €
4802 Isolation of DNA/RNA from cells-Mini
100 samples 20,00 €
4803 Pesteling of probes
100 samples 15,00 €
4804 RNA extraction
100 samples 10,00 €
4805 Probe preparation for extraction
100 samples 5,00 €
4820 Agarose gel purification
1 sample 50,00 €
4821 Agarose gel purification
>10 samples 20,00 €
4830 Spin column purification
1 sample 10,00 €
4840 Restriction digest
1 sample 20,00 €
4845 UV measurement at 260 nm and DNA adjustment
1 sample 10,00 €
4850 UV measurement at 260 nm
1 sample 5,00 €
4855 Adjustment of DNA to 5 ng/µl
1 sample 100,00 €
4870 Dilution of DNA to 1x106 copys
each sample 100,00 €
4880 Filling in tubes
each sample 20,00 €
4890 Storage of the DNA
each sample 100,00 €
4860 Agarose gel analysis
1 sample 10,00 €
4880 Removal of genomic E. coli DNA from Maxiprep
1 sample 200,00 €
4900 E. coli Miniprep
1 sample 20,00 €
4901 E. coli Miniprep
2-10 samples 15,00 €
4902 E. coli Miniprep
>10 samples 10,00 €
4905 Colony PCR
>10 samples 5,00 €
4910 Restriction and religation for Maxiprep
1 sample 100,00 €
4920 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 1 sample 140,00 €
4921 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 2 samples 130,00 €
4922 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 3 samples 125,00 €
4923 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 4 samples 120,00 €
4924 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 6 samples 115,00 €
4925 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 8 samples 110,00 €
4926 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 12 samples 105,00 €
4927 Isolation of Maxi-Prep DNA (up to 500 µg) high copy 16 samples 100,00 €
4930 Isolation of Mega-Prep DNA (up to 2.000 µg)
1 sample 400,00 €
4931 Isolation of Mega-Prep DNA (up to 2.000 µg)
2 samples 350,00 €
4940 Isolation of Giga-Prep DNA (up to 10.000 µg)
1 sample 1.500,00 €
4950 Isolation of 60mg DNA
1 sample 4.000,00 €
4960 Isolation of Maxi-Prep DNA (up to 200 µg) low copy 1 sample 195,00 €
4961 Isolation of Maxi-Prep DNA (up to 200 µg) low copy 2 samples 180,00 €
4962 Isolation of Maxi-Prep DNA (up to 200 µg) low copy 4 samples 165,00 €
4963 Isolation of Maxi-Prep DNA (up to 200 µg) low copy 8 samples 150,00 €
4970 Isolation of Maxi-Prep DNA (up to 500 µg) Animal component free 1 sample 250,00 €
4971 Isolation of Maxi-Prep DNA (up to 500 µg) Animal component free 2 sample 220,00 €
4972 Isolation of Maxi-Prep DNA (up to 500 µg) Animal component free 3 sample 200,00 €






G. Sequencing price per sample (average reading length: 20-1000bp/99%)


5105 Sequencing primer
1 primer 20,00 €
5106 Primer for Gene synthesis (30 bp)
1 primer 30,00 €
5110 Column DNA purification and sequencing
100 samples 6,50 €
5120 Sequencing: normal
11-100 samples 9,00 €
5130 Sequencing: normal
101-1000 samples 8,00 €
5410 Sequencing and comparison with a known sequence or storage of the sequence
each sample 20,00 €
5415 Comparison with a known sequence
each sample 5,00 €
5421 Full documentation of the DNA in gene bank format

50,00 €
5422 Sequencing of unknown DNA and documentation
1000 bp 100,00 €
5430 Import of sequences from NCBI

10,00 €
5510 DNA isolation, amplification and sequencing of genes from genomic DNA (16S-RNA, rubisco etc.)

200,00 €






H. Supercompetent cells from your E. coli strain


6110 Preparation of supercompetent cells from your E. coli strain
in 2 ml vials (Transformation rate in 2ml vials: 1x109)

128 vials 384,00 €
6112 Preparation of supercompetent cells from your E. coli strain
in 2 ml vials (Transformation rate in 2ml vials: 1x109)

64 vials 192,00 €






I. Plant transformation


7001 Transformation into Arabidopsis

500,00 €
7002 Transformation into Arabidopsis (T1 seeds tested)

750,00 €
7003 Transformation into Arabidopsis (T2 seeds)

1.250,00 €





8000 mRNA-synthesis with capping and Tailing from Plasmid, 50 µg

450,00 €
8001 mRNA-synthesis with capping and Tailing from Plasmid, 100 µg

1.000,00 €
8002 mRNA-synthesis with capping and Tailing from Plasmid, 150 µg

1.200,00 €
8003 mRNA-synthesis with capping and Tailing from Plasmid, 200 µg

1.400,00 €
8004 mRNA-synthesis with capping and Tailing from Plasmid, 300 µg

1.800,00 €
8005 mRNA-synthesis with capping and Tailing from Plasmid, 400 µg

2.200,00 €
8006 mRNA-synthesis with capping and Tailing from Plasmid, 600 µg

3.000,00 €






Setup costs:



When amplification failed:

costs for primer

When cloning failed: Due to our experience 99% of our cloning experiments are succesful

50 % of cloning costs






miscellaneous


10001 Delivery on ice

30,00 €
10001 Delivery on dry ice

20,00 €
10002 Delivery with FedEx

50-100 €
10003 Delivery with Expeditors
150,00 €
10004 payment with cheque

10,00 €
10005 payment with PayPal in Euro

5%
10006 PayPal payment in other currencies

10%
10006 payment with wire transfer

free
10007 Export handling

50,00 €
10008 Special Invoice Handling

50,00 €
10009 Additional Cost estimation

50,00 €






General recommendations



Prices are without 19% VAT

If you send us plasmid DNA for cloning, we prefer it purified by a midi/maxi prep in TE-buffer

We need a minimum of 2µg vector DNA at a minimum concentration of 200ng/µl

Other plasmids should be already transformed in E. coli on a agar plate.

As a host strain we prefer DH5-alpha, XL1-blue or JM109

Generel cloning conditions will be valid for restriction enzymes with an overhang of

at least 3 bases, DNA fragments up to 2.000 bp and high-copy plasmids.

We don´t take any guaranty for genomic sequences

DNA data should be readable by a computer
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