DNA Extraction/Genomic DNA Purification
The Genomic DNA Mini Kit (Plant) is a genomic DNA extraction kit optimized for genomic DNA, mitochondrial DNA and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates.
In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 30 minutes. For RNA purification from plant samples, the Total RNA Mini Kit (Plant) is also available.
220,00 € excl. VAT
PCR, Real-time PCR, Southern Blotting and RFLP
The quality of the Genomic DNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 50 mg fresh young leaf samples. The purified genomic DNA (>10 µg) is quantified with a spectrophotometer and checked by electrophoresis.
Plant SpeciesnDNA Yield
| Thale Cress (Arabidopsis thaliana) | 3~5 µg |
| Rice (Oryza sativa) | 10~15 µg |
| Tomato (Lycopersicon esculentum) | 10~15 µg |
| Chinese Yam (Rhizoma dioscoreae) | 30~60 µg |
| Com (Zea mays) | 15~20 µg |
| Sweet Potato (lpomoea batatas) | 20~30 µg |
| Orchid (Phalaenopsis aphrodite) | 5~10 µg |
| Camphor (Cinnamommun camphora) | 15~20 µg |
| Spinach (Spinacia oleracea) | 5~10 µg |
| Bamboo (Bambusa iodhamii) | 10~15 µg |
Table 1. The approximate genomic DNA yield from a variety of plant species using the Genomic DNA Mini Kit (Plant).
| Specifications | gSYNC™ DNA Extraction Kit | Genomic DNA Mini Kit Blood Cultured Cell | Genomic DNA Mini Kit Tissue | Genomic DNA Mini/Maxi Kit Plant | Presto™ Mini gDNA Bacteria Kit | Presto™ Mini gDNA Yeast Kit | Geneius™ Micro gDNA Extraction Kit | Presto™ Buccal Swab gDNA Extraction Kit |
|---|---|---|---|---|---|---|---|---|
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM020 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK10 GSK300 |
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column |
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg |
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs |
| 20 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||
| hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A |
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes |
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | N/A | Proteinase K | Proteinase K |
| Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab |
| 10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 30-200 µl | 30-100 µl | 50-100 µl |
| Operation Time | <20 minutes | <20 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
