Plant Genomic DNA Mini Kit

DNA Extraction / Genomic DNA Purification

The Genomic DNA Mini Kit (Plant) is a genomic DNA extraction kit optimized for genomic DNA, mitochondrial DNA and chloroplast DNA purification from plant tissue. This DNA extraction kit processes plant samples by grinding in liquid nitrogen followed by lysis buffer incubation. The lysate is treated with RNase A then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. DNA phenol extraction or alcohol precipitation is not required, and the entire procedure can be completed in less than 30 minutes. For RNA purification from plant samples, the Total RNA Mini Kit (Plant) is also available.

220,00  excl. VAT

DESCRIPTION

Specifications (Cat. # GP100)

  • Purify genomic DNA in less than 30 minutes!
  • High Yield: 3-5 μg (100 mg Thale cress), 20-25 µg (100 mg Tobacco)
  • Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-200 µl
  • Storage: dry at room temperature (15-25°C)

Applications

PCR, Real-time PCR, Southern Blotting and RFLP

Components

  • GPX1 Buffer (optimized for samples with high polysaccharide content)
  • GP1, GP2, GP3 Buffers
  • RNase A
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Filter Column
  • GD Column
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Genomic DNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is extracted from 50 mg fresh young leaf samples. The purified genomic DNA (>10 µg) is quantified with a spectrophotometer and checked by electrophoresis.

Genomic DNA Mini Kit (Plant) Interactive Protocol

Figure 1. Genomic DNA was extracted from a variety of 100 mg plant sample species using the Genomic DNA Mini Kit (Plant). A 3 µl aliquot from each 200 µl eluate was analyzed by electrophoresis on a 1% agarose gel.
M = Geneaid 1 Kb DNA Ladder
  1. Cinnamomun camphora (Camphor tree)
  2. Pisum sativum (Pea sprout)
  3. Arabidopsis thaliana (Thale cress)
  4. Oryza sativa (Rice)
  5. Ipomoea batatas (Sweet potato vine)
  6. Rhizoma dioscoreae (Chinese yam )
  7. Populus tremula (Aspen)
  8. Flammulina velutipes (Mushroom)
  9. Oxalis comiculats (Clover)
Plant SpeciesnDNA Yield

Thale Cress (Arabidopsis thaliana) 3~5 µg
Rice (Oryza sativa) 10~15 µg
Tomato (Lycopersicon esculentum) 10~15 µg
Chinese Yam (Rhizoma dioscoreae) 30~60 µg
Com (Zea mays) 15~20 µg
Sweet Potato (lpomoea batatas) 20~30 µg
Orchid (Phalaenopsis aphrodite) 5~10 µg
Camphor (Cinnamommun camphora) 15~20 µg
Spinach (Spinacia oleracea) 5~10 µg
Bamboo (Bambusa iodhamii) 10~15 µg

Table 1. The approximate genomic DNA yield from a variety of plant species using the Genomic DNA Mini Kit (Plant).

Genomic DNA Extraction Kits Specifications

Specifications gSYNC™ DNA Extraction Kit Genomic DNA Mini Kit Blood Cultured Cell Genomic DNA Mini Kit Tissue Genomic DNA Mini/Maxi Kit Plant Presto™ Mini gDNA Bacteria Kit Presto™ Mini gDNA Yeast Kit Geneius™ Micro gDNA Extraction Kit Presto™ Buccal Swab gDNA Extraction Kit
Cat. # GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM020
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK10
GSK300
Format Spin Column Spin Column Spin Column Spin Column Spin Column Spin Column Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg (Mini)
500 µg (Maxi)
50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
20 mg animal tissue 200 µl buffy coat Maxi: 1 g plant tissue 200 µl blood FTA® Cards
1x107 cultured cells 1x107 cultured cells 1 ml urine, plasma 1 ml urine
hair 2x108 yeast/fungus 1-100 µl saliva
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme Proteinase K N/A Proteinase K N/A Lysozyme Proteinase K N/A Proteinase K Proteinase K
Typical Yield 5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini) 37 µg from 1x109 E. coli 10 µg from 2x108 S. cerevisiae cells 300 ng from 6 mm blood spots 2 µg per swab
10-40 µg from
20 mg tissue
80 µg from 500 mg Lemon leaf (Maxi) 10-15 µg from 1x109 Bacillus subtilis
Elution Volume 30-100 µl 30-200 µl 30-200 µl 30-200 µl (Mini)
1 ml (Maxi)
30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <20 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
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