The GenepHlow™ Gel Extraction Kit and PCR Cleanup Kit was designed to recover or concentrate DNA fragments from agarose gel, PCR or other enzymatic reactions. This gel extraction kit and PCR cleanup kit includes a pH indicator which is premixed with the binding buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow.
3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.
100,00 € – 250,00 € excl. VAT
Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection
The quality of the GenepHlow™ Gel/PCR Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions analyzed by electrophoresis.
Figure 1 .Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel/PCR Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel.M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1100 ng)
Figure 2 .PCR product DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel/PCR Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1073 ng)
|Specifications||GenepHlow™ Gel Extraction Kit||GenepHlow™ PCR Cleanup Kit||GenepHlow™ Gel/PCR Kit||GenepHlow™ DNA Cleanup Maxi Kit||Presto™ 96 Well Gel Extraction Kit||Small DNA Fragments Extraction Kit||Large DNA Fragments Extraction Kit|
|Format||Spin Column||Spin Column||Spin Column||Maxi Spin Column||96 Well Plate||Spin Column||Silica Matrix|
|Binding Capacity||10 µg||10 µg||10 µg||200 µg||10 µg||10 µg||5 µg|
|Sample||Agarose Gel Slice||PCR Product||Agarose Gel Slice/PCR Product||Agarose Gel Slice/Restriction Enzyme Digest Product||Agarose Gel Slice||Agarose Gel Slice/PCR Product||Agarose Gel Slice/PCR Product|
|Fragment Size||70 bp-20 kb||70 bp-20 kb||70 bp-20 kb||100 bp-10 kb||70 bp-20 kb||40-200 bp||100 bp-50 kb|
|Recovery||Up to 90%||Up to 95%||Up to 95%||Up to 80%||Up to 80%||Up to 95%||Up to 95% (<10 kb)|
Up to 80% (10-50 kb)
|Elution Volume||20-50 µl||20-50 µl||20-50 µl||500-1000 µl||60-80 µl||20-50 µl||10-20 µl|
|Operation Time||20 minutes||10 minutes||<20 minutes||<25 minutes||45 minutes||<20 minutes||<20 minutes|