GenepHlow Gel/PCR Kit

Specifications (Cat. # DFH100, DFH300)

• High Efficiency: up to 90% recovery from agarose gels, up to 95% recovery from PCR or other enzymatic reactions
• Only 20 minute gel extraction and 10 minute PCR cleanup
• Convenient: Gel/PCR binding buffer is premixed with a pH indicator to allow for easy determination of optimal pH
• Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
• Sample Size: up to 300 mg of agarose gel slice or up to 100 μl of PCR products
• Broad Fragment Size Range: 70 bp–20 kb
• Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup
• Elution Volume: 20-50 µl
• Storage: dry at room temperature (15-25°C)

Applications

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

Components

• Gel/PCR Buffer (premixed with a pH indicator)
• 3M Sodium Acetate (pH5.0)
• W1 Buffer
• Wash Bufffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
• DFH Columns
• 2 ml Collection Tubes

QUALITY CONTROL

The quality of the GenepHlow™ Gel/PCR Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions analyzed by electrophoresis.

GenepHlow™ Gel/PCR Kit Functional Test Data

Figure 1 .Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel/PCR Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel.M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1100 ng)

TestDNA Conc.260/280YieldRecovery

1	18.2 ng/µl	1.84	910ng	82%
2	18.7 ng/µl	1.82	935ng	85%
3	19.2 ng/µl	1.82	960ng	87.3%

Figure 2 .PCR product DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel/PCR Kit (lane 1, 2, 3). The purified DNA from a 50 µl eluate was confirmed by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1073 ng)

TestDNA Conc.260/280YieldRecovery

1	19.7 ng/µl	1.86	985ng	91.8%
2	20.3 ng/µl	1.85	1015ng	94.6%
3	20.5 ng/µl	1.86	1025ng	95.5%

Figure 3. A pH indicator is premixed with the binding buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.