Agrobacterium Strains

We only work with strain GV3101-pMP90RK.
This strain works excellent with all dicots. People have also very succesful transformed Brachypodium and other monocots with this strain.

Agrobacterium Strain Chromosomal BackgroundMarker Gene Ti PLasmid Marker Gene Opine
LBA4404TiAch5rifpAL4404spec and strepOctopine
GV2260C58rifpGV2260 (pTiB6S3DT-DNA)carbOctopine
A136C58rif and nalCuredNopaline
GV3101::pMP90C58rifpMP90 (pTiC58DT-DNA)gentNopaline
GV3101::pMP90RKC58rifpMP90RK (pTiC58DT-DNA)gent and kanNopaline
EHA101C58rifpEHA101 (pTiBo542DT-DNA)kanNopaline
EHA105C58rifpEHA105 (pTiBo542DT-DNA)Succinamopine
AGL-1C58, RecArif, carbpTiBo542DT-DNASuccinamopine

General recommendations for plant transformation with binaries.

  • For transformation of dicots we recommend GV3101-pMP90RK and EHA105. We can not recommend LBA4404, though some people are also succesful with this strain.
  • For transformation of Brachypopdium we can also recommend GV3101-pMP90RK.
  • For transformation of other monocots AGL1 and LBA4404 are often used. There exist strains of LBA4404 without and with a strep/spec resistance.
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